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KMID : 0379120030310020077
Korean Journal of Mycology
2003 Volume.31 No. 2 p.77 ~ p.83
Studies on the Cultural Characteristics of Cellulase Production by Roseofomes subflexibilis
Jang Hyung-Soo

Abstract
For the purpose of utilizing cellulose resources by cellulolytic enzymes of Roseofomes subflexibilis, it¡¯s cultural conditions for the production of cellulolytic enzymes in synthetic media were investigated. The results obtained were summarized as follows : The optimum temperature and pH for the enzyme production were $30^{\circ}C$ and pH 4.0, respectively. Among the carbon sources, glucose was good for the production of cellulase. The optimum concentration of saccharose as 1.2%. As a organic nitrogen source, yeast extract was good for the mycelial growth. The optimum concentration of yeast extract as 1.5%. As a inorganic nitrogen source, $NH_{4}H_{2}PO_{4}$ was good for the mycelial growth. The optimum concentration of $NH_{4}H_{2}PO_{4}$ were 1.1%. The mineral salt of $Al_{2}(SO_{4})_{3}$ was effective and the optimum concentration was 0.1 M.
KEYWORD
Cellulase, Roseofomes subflexibilis
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